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This reduces the partial stress of oxygen, decreasing its saturation levels and contributing to hypoxic (low O2) conditions ³⁵. Chen et al. (1993 ) reported that mutants of R. leguminosarum have been in a position to grow at a pH as little as 4.5. Moreover, Foster (2000 ) recorded that S. meliloti was viable solely below to pH 5.5. Some rhizobia have wide selection of pH similar to S. fredii can grow properly between pH four – 9.5 however B. japonicum can not grow at the extremes of that range ( Fujihara and Yoneyama 1993 ). These values are the extremes and hindered the expansion and survival of micro organism between 1 and a couple of pH items (

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